Microspore Culture and Hairy Root Transformation

Pollen or microspore culture is an in vitro technique by which the pollen grains, preferably at the uni-nucleated stage, are squeezed out asep­tically from the intact anther and then cultured on nutrient medium where the microspores, without producing male gametes, develop into haploid embryoids or callus tissue that give rise to haploid plantlets by embryogenesis or orga­nogenesis.