Plant Genomics

Biography

FahadAl-Qurainy is the professor, expertise in Department of Botany and Microbiology at King Saud University. He publishes more than 60 articles related to plants.

Abstract

The cryopreservation of pre-embryonic callus of date palm (Phoenix dactylifera L.) cv. Sagai investigated through encapsulation-dehydration, vitrification and encapsulation -vitrification the maximum regrowth (53.33%) of encapsulated cryopreserved (+LN) embryogenic calli was noted when pre-embryos mass was incubated with 0.5 M of sucrose for two days followed by 6 h air dehydration. The greatest survival (80%) of encapsulated cryopreserved embryonic clump was resulted when calli were incubated with 0.3 or 0.7 M of sucrose for two days followed by 4 h of dehydration, or with 0.5 M of sucrose for two days without air dehydration or 2 h of dehydration. After cryopreservation using encapsulation-vitrification protocol, the highest survival rate (86.67%) and the maximum regrowth (46.67%) achieved when the encapsulated-vitrified cryopreserved calli treated with 100% plant vitrification solution 2 (PVS2) at 25ËšC for 60 min. After cryopreservation using vitrification protocol, the highest recovery 53.33% attained when the vitrified cryopreserved embryogenic calli treated with PVS2 at 25ËšC for 30 min. The maximum (40%) regrowth of vitrified cryopreserved embryogenic calli observed when the vitrified cryopreserved embryogenic calli subjected to PVS2 at 25ËšC for 60 min. The results obtained during this study for regrowth after cryopreservation of Sagai were above the minimum expected for a cryopreserved germplasm bank. The regeneration and regrowth were more than 30% in all the methods applied for Sagai.